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Muscarinic AChR M2 (ACM2) Rabbit pAb (A1567)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Muscarinic AChR M2 (ACM2) Rabbit pAb (A1567)

Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (ACM2) antibody (A1567) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameMuscarinic AChR M2 (ACM2) Rabbit pAb
Catalog No.A1567
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 208-388 of human Muscarinic AChR M2 (ACM2) (NP_001006629.1).
SequenceHISRASKSRIKKDKKEPVANQDPVSPSLVQGRIVKPNNNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKSDSCTPTNTTVEVVGSSGQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKKVTRT
Gene ID
Swiss Prot
SynonymsHM2
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSH-SY5Y, HepG2
Cellular locationCell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Customer validation

IHC(Homo sapiens)

Documents

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ABclonal:Western blot - Muscarinic AChR M2 (ACM2) Rabbit pAb (A1567)}

Western blot - Muscarinic AChR M2 (ACM2) Rabbit pAb (A1567)

Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (ACM2) antibody (A1567) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1567? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)