제품 > 항체 > 다중클론항체(pAb)

Myod1 Rabbit pAb (A23881)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from Mouse skeletal muscle, using Myod1 Rabbit pAb (A23881) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Myod1 Rabbit pAb
Catalog No.	A23881
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables several functions, including DNA binding activity; DNA-binding transcription activator activity, RNA polymerase II-specific; and promoter-specific chromatin binding activity. Contributes to DNA binding activity. Involved in several processes, including cellular response to estradiol stimulus; histone acetylation; and positive regulation of snRNA transcription by RNA polymerase II. Acts upstream of or within several processes, including myotube differentiation; regulation of RNA metabolic process; and skeletal muscle fiber adaptation. Located in euchromatin; myofibril; and nucleus. Part of transcription regulator complex. Is expressed in several structures, including alimentary system; embryo mesenchyme; limb; limb bud; and musculature. Orthologous to human MYOD1 (myogenic differentiation 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of mouse Myod1 (NP_034996.2)
Sequence	MELLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRFFEDLDPRLVHMGALLKPEEHAHFPTAVHPGPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAFYAPGPLPPGRGSEHYSGDSDASSPRSNCSDGMMDYSGPPSGPRRQNGYDTAYYSEAARESRPGKSAAVSSLDCLSSIVERISTDSPAAPALLLADAPPESPPGPPEGASLSDTEQGTQTPSPDAAPQCPAGSNPNAIYQVL
Gene ID
Swiss Prot
Synonyms	MYF3; MyoD; Myod-1; bHLHc1; Myod1
Calculated MW	34KDa
Observed MW	45kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse skeletal muscle
Cellular location