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Myogenin Rabbit pAb (A6664)

Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Myogenin Rabbit pAb (A6664)

Western blot analysis of lysates from Rat skeletal muscle, using Myogenin Rabbit pAb (A6664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Myogenin Rabbit pAb (A6664)

Western blot analysis of various lysates, using Myogenin Rabbit pAb (A6664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): 293T, NIH/3T3
Exposure time: 30s.

Overview

Product nameMyogenin Rabbit pAb
Catalog No.A6664
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human Myogenin (NP_002470.2).
SequenceMELYETSPYFYQEPRFYDGENYLPVHLQGFEPPGYERTELTLSPEAPGPLEDKGLGTPEHCPGQCLPWACKVCKRKSVSVDRRRAATLREKRRLKKVNEAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLNQEERDLRYRGGGGPQPGVPSECSSHSASCSPEWGSALEFSANPGDHLLTADPTDAHNLHSLTSIVDSITVEDVSVAFPDETMPN
Gene ID
Swiss Prot
SynonymsMYF4; myf-4; bHLHc3; Myogenin
Calculated MW25kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat skeletal muscle, RD, Mouse skeletal muscle, Rat heart
Cellular locationNucleus
Customer validation

WB(Mus musculus, Bos taurus, Rattus norvegicus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Myogenin Rabbit pAb (A6664)}

Western blot - Myogenin Rabbit pAb (A6664)

Western blot analysis of lysates from Rat skeletal muscle, using Myogenin Rabbit pAb (A6664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Myogenin Rabbit pAb (A6664)}

Western blot - Myogenin Rabbit pAb (A6664)

Western blot analysis of various lysates, using Myogenin Rabbit pAb (A6664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): 293T, NIH/3T3
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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