제품 > 항체 > 다중클론항체(pAb)

NAE1 Rabbit pAb (A4254)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NAE1 Rabbit pAb (A4254)

Western blot analysis of various lysates using NAE1 Rabbit pAb (A4254) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameNAE1 Rabbit pAb
Catalog No.A4254
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NAE1 (NP_003896.1).
SequenceMAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAK
Gene ID
Swiss Prot
SynonymsHPP1; ula-1; APPBP1; NEDFIH; A-116A10.1; NAE1
Calculated MW60kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU2OS, OVCAR3, PC-3, Mouse liver, Rat brain
Cellular locationCell membrane.

Documents

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    ABclonal:Western blot - NAE1 Rabbit pAb (A4254)}

    Western blot - NAE1 Rabbit pAb (A4254)

    Western blot analysis of various lysates using NAE1 Rabbit pAb (A4254) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)