제품 > 항체 > 다중클론항체(pAb)

NASP Rabbit pAb (A6938)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NASP Rabbit pAb (A6938)

Western blot analysis of various lysates using NASP Rabbit pAb (A6938) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - NASP Rabbit pAb (A6938)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using NASP Rabbit pAb (A6938) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NASP Rabbit pAb (A6938)

Immunohistochemistry analysis of paraffin-embedded Human brain astrocytoma using NASP Rabbit pAb (A6938) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NASP Rabbit pAb (A6938)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using NASP Rabbit pAb (A6938) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameNASP Rabbit pAb
Catalog No.A6938
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
SequenceWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC
Gene ID
Swiss Prot
SynonymsHMDRA1; FLB7527; PRO1999; NASP
Calculated MW85kDa
Observed MW85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSW620, HL-60, 293T
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - NASP Rabbit pAb (A6938)}

Western blot - NASP Rabbit pAb (A6938)

Western blot analysis of various lysates using NASP Rabbit pAb (A6938) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - NASP Rabbit pAb (A6938)}

Immunohistochemistry - NASP Rabbit pAb (A6938)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using NASP Rabbit pAb (A6938) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NASP Rabbit pAb (A6938)}

Immunohistochemistry - NASP Rabbit pAb (A6938)

Immunohistochemistry analysis of paraffin-embedded Human brain astrocytoma using NASP Rabbit pAb (A6938) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NASP Rabbit pAb (A6938)}

Immunohistochemistry - NASP Rabbit pAb (A6938)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using NASP Rabbit pAb (A6938) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)