Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using NCAPD2 Rabbit pAb (A18412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunofluorescence analysis of C6 cells using NCAPD2 Rabbit pAb (A18412) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NCAPD2 Rabbit pAb (A18412) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | NCAPD2 Rabbit pAb |
|---|---|
| Catalog No. | A18412 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1250-1350 of human NCAPD2 (NP_055680.3). |
|---|---|
| Sequence | FGDKLSDESIFSAFLSVVGKLRRGAKPEGKAIIDEFEQKLRACHTRGLDGIKELEIGQAGSQRAPSAKKPSTGSRYQPLASTASDNDFVTPEPRRTTRRHP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CNAP1; CAP-D2; MCPH21; hCAP-D2; NCAPD2 |
| Calculated MW | 157kDa |
| Observed MW | 175kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | Raji, K-562, HeLa, Mouse liver, Rat liver |
| Cellular location | condensed nuclear chromosome, cytoplasm, cytosol, nucleoplasm, nucleus |
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* For research use only. Not for therapeutic or diagnostic purposes.
