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NCK1 Rabbit mAb (A9129)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NCK1 Rabbit mAb (A9129)

Western blot analysis of various lysates using NCK11 Rabbit mAb (A9129) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - NCK1 Rabbit mAb (A9129)

Immunohistochemistry analysis of paraffin-embedded Rat testis using NCK11 Rabbit mAb (A9129) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NCK1 Rabbit mAb (A9129)

Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (A9129) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNCK1 Rabbit mAb
Catalog No.A9129
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1441
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human NCK11 (P16333).
SequencePFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERG
Gene ID
Swiss Prot
SynonymsNCK; nck-1; NCKalpha; NCK1
Calculated MW43kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, Mouse lung, Mouse brain, Rat brain
Cellular locationCytoplasm, Endoplasmic reticulum, Nucleus
Customer validation

IF(Mus musculus)

Documents

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ABclonal:Western blot - NCK1 Rabbit mAb (A9129)}

Western blot - NCK1 Rabbit mAb (A9129)

Western blot analysis of various lysates using NCK11 Rabbit mAb (A9129) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - NCK1 Rabbit mAb (A9129)}

Immunohistochemistry - NCK1 Rabbit mAb (A9129)

Immunohistochemistry analysis of paraffin-embedded Rat testis using NCK11 Rabbit mAb (A9129) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NCK1 Rabbit mAb (A9129)}

Immunofluorescence - NCK1 Rabbit mAb (A9129)

Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (A9129) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)