제품 > 항체 > 다중클론항체(pAb)

NCOA3 Rabbit pAb (A17330)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - NCOA3 Rabbit pAb (A17330)

Western blot analysis of lysates from HeLa cells, using NCOA3 Rabbit pAb (A17330) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunoprecipitation - NCOA3 Rabbit pAb (A17330)

Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg NCOA3 Rabbit pAb (A17330). Western blot was performed from the immunoprecipitate using NCOA3 Rabbit pAb (A17330) at a dilition of 1:2000.

Overview

Product nameNCOA3 Rabbit pAb
Catalog No.A17330
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human NCOA3 (NP_858045.1).
SequenceMSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADV
Gene ID
Swiss Prot
SynonymsACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42; NCOA3
Calculated MW155kDa
Observed MW160kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesHeLa
Cellular locationcytoplasm, cytosol, extracellular exosome, nucleoplasm, nucleus.

Documents

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ABclonal:Western blot - NCOA3 Rabbit pAb (A17330)}

Western blot - NCOA3 Rabbit pAb (A17330)

Western blot analysis of lysates from HeLa cells, using NCOA3 Rabbit pAb (A17330) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunoprecipitation - NCOA3 Rabbit pAb (A17330)}

Immunoprecipitation - NCOA3 Rabbit pAb (A17330)

Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg NCOA3 Rabbit pAb (A17330). Western blot was performed from the immunoprecipitate using NCOA3 Rabbit pAb (A17330) at a dilition of 1:2000.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)