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NCOR2 Rabbit pAb (A8388)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NCOR2 Rabbit pAb (A8388)

Western blot analysis of various lysates using NCOR2 Rabbit pAb (A8388) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

ABclonal:Immunohistochemistry - NCOR2 Rabbit pAb (A8388)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using NCOR2 Rabbit pAb (A8388) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NCOR2 Rabbit pAb (A8388)

Immunohistochemistry analysis of paraffin-embedded Human colon using NCOR2 Rabbit pAb (A8388) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NCOR2 Rabbit pAb (A8388)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using NCOR2 Rabbit pAb (A8388) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameNCOR2 Rabbit pAb
Catalog No.A8388
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NCOR2 (NP_006303.4).
SequenceMSGSTQPVAQTWRATEPRYPPHSLSYPVQIARTHTDVGLLEYQHHSRDYASHLSPGSIIQPQRRRPSLLSEFQPGNERSQELHLRPESHSYLPELGKSEMEFIESKRPRLELL
Gene ID
Swiss Prot
SynonymsSMRT; TRAC; CTG26; SMRTE; TRAC1; N-CoR2; TNRC14; TRAC-1; SMAP270; SMRTE-tau; NCOR2
Calculated MW274kDa
Observed MW275kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, LO2
Cellular locationNucleus

Documents

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ABclonal:Western blot - NCOR2 Rabbit pAb (A8388)}

Western blot - NCOR2 Rabbit pAb (A8388)

Western blot analysis of various lysates using NCOR2 Rabbit pAb (A8388) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Immunohistochemistry - NCOR2 Rabbit pAb (A8388)}

Immunohistochemistry - NCOR2 Rabbit pAb (A8388)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using NCOR2 Rabbit pAb (A8388) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NCOR2 Rabbit pAb (A8388)}

Immunohistochemistry - NCOR2 Rabbit pAb (A8388)

Immunohistochemistry analysis of paraffin-embedded Human colon using NCOR2 Rabbit pAb (A8388) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NCOR2 Rabbit pAb (A8388)}

Immunohistochemistry - NCOR2 Rabbit pAb (A8388)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using NCOR2 Rabbit pAb (A8388) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)