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NDUFA1 Rabbit mAb (A20940)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - NDUFA1 Rabbit mAb (A20940)

Western blot analysis of various lysates using NDUFA1 Rabbit mAb (A20940) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - NDUFA1 Rabbit mAb (A20940)

Immunohistochemistry analysis of paraffin-embedded human liver using NDUFA1 Rabbit mAb (A20940) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NDUFA1 Rabbit mAb (A20940)

Immunohistochemistry analysis of paraffin-embedded human small intestine using NDUFA1 Rabbit mAb (A20940) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameNDUFA1 Rabbit mAb
Catalog No.A20940
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2926
The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-70 of human NDUFA1 (O15239).
SequenceMWFEILPGLSVMGVCLLIPGLATAYIHRFTNGGKEKRVAHFGYHWSLMERDRRISGVDRYYVSKGLENID
Gene ID
Swiss Prot
SynonymsMWFE; ZNF183; CI-MWFE; MC1DN12; NDUFA1
Calculated MW8kDa
Observed MW10kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562, U-2 OS
Cellular locationMitochondrion inner membrane, Single-pass membrane protein
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - NDUFA1 Rabbit mAb (A20940)}

Western blot - NDUFA1 Rabbit mAb (A20940)

Western blot analysis of various lysates using NDUFA1 Rabbit mAb (A20940) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - NDUFA1 Rabbit mAb (A20940)}

Immunohistochemistry - NDUFA1 Rabbit mAb (A20940)

Immunohistochemistry analysis of paraffin-embedded human liver using NDUFA1 Rabbit mAb (A20940) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NDUFA1 Rabbit mAb (A20940)}

Immunohistochemistry - NDUFA1 Rabbit mAb (A20940)

Immunohistochemistry analysis of paraffin-embedded human small intestine using NDUFA1 Rabbit mAb (A20940) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)