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NDUFAF1 Rabbit mAb (A4007)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - NDUFAF1 Rabbit mAb (A4007)

Western blot analysis of extracts of various cell lines, using NDUFAF1 Rabbit mAb (A4007) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - NDUFAF1 Rabbit mAb (A4007)

Immunofluorescence analysis of HeLa cells using NDUFAF1 Rabbit mAb (A4007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameNDUFAF1 Rabbit mAb
Catalog No.A4007
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2091
This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFAF1 (Q9Y375).
SequenceMALVHKLLRGTYFLRKFSKPTSALYPFLGIRFAEYSSSLQKPVASPGKASSQRKTEGDLQGDHQKEVALDITSSEEKPDVSFDKAIRDEAIYHFRLLKDEIVDHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEV
Gene ID
Swiss Prot
SynonymsCGI65; CIA30; CGI-65; MC1DN11
Calculated MW38kDa
Observed MW35kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU2OS, 293T, HepG2
Cellular locationcytosol, mitochondrial inner membrane, mitochondrial matrix, mitochondrial respiratory chain complex I, mitochondrion

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ABclonal:Western blot - NDUFAF1 Rabbit mAb (A4007)}

Western blot - NDUFAF1 Rabbit mAb (A4007)

Western blot analysis of extracts of various cell lines, using NDUFAF1 Rabbit mAb (A4007) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - NDUFAF1 Rabbit mAb (A4007)}

Immunofluorescence - NDUFAF1 Rabbit mAb (A4007)

Immunofluorescence analysis of HeLa cells using NDUFAF1 Rabbit mAb (A4007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)