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NDUFC2 Rabbit pAb (A3312)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NDUFC2 Rabbit pAb (A3312)

Western blot analysis of lysates from A-549 cells, using NDUFC2 Rabbit pAb (A3312).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameNDUFC2 Rabbit pAb
Catalog No.A3312
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in mitochondrial respiratory chain complex I assembly. Located in mitochondrion. Part of mitochondrial respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1).
SequenceMIARRNPEPLRFLPDEARSLPPPKLTDPRLLYIGFLGYCSGLIDNLIRRRPIATAGLHRQLLYITAFFFAGYYLVKREDYLYAVRDREMFGYMKLHPEDF
Gene ID
Swiss Prot
SynonymsHLC-1; B14.5b; MC1DN36; NADHDH2; CI-B14.5b; NDUFC2
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549
Cellular locationMatrix side, Mitochondrion inner membrane, Single-pass membrane protein

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ABclonal:Western blot - NDUFC2 Rabbit pAb (A3312)}

Western blot - NDUFC2 Rabbit pAb (A3312)

Western blot analysis of lysates from A-549 cells, using NDUFC2 Rabbit pAb (A3312).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)