제품 > 항체 > 다중클론항체(pAb)

NDUFS7 Rabbit pAb (A24466)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - NDUFS7 Rabbit pAb (A24466)

Western blot analysis of various lysates, using NDUFS7 Rabbit pAb (A24466) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameNDUFS7 Rabbit pAb
Catalog No.A24466
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH):ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 80-160 of human NDUFS7 (NP_077718.3).
SequenceWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYY
Gene ID
Swiss Prot
SynonymsNDUFS7; CI-20; CI-20KD; MY017; PSST; NADH:ubiquinone oxidoreductase core subunit S7
Calculated MW22kDa/23kDa
Observed MW20kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA549, HeLa, 293T
Cellular locationMitochondrion
Customer validation

WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - NDUFS7 Rabbit pAb (A24466)}

Western blot - NDUFS7 Rabbit pAb (A24466)

Western blot analysis of various lysates, using NDUFS7 Rabbit pAb (A24466) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)