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NEDD4L Rabbit mAb (A9078)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - NEDD4L Rabbit mAb (A9078)

Western blot analysis of extracts of various cell lines, using NEDD4L antibody (A9078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameNEDD4L Rabbit mAb
Catalog No.A9078
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1404
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 876-975 of human NEDD4L (Q96PU5).
SequenceNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD
Gene ID
Swiss Prot
SynonymsRSP5; PVNH7; NEDD4-2; NEDD4.2; hNEDD4-2
Calculated MW112kDa
Observed MW135kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, K-562
Cellular locationCytoplasm, cytosol, extracellular exosome, Golgi apparatus, multivesicular body, nucleoplasm
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - NEDD4L Rabbit mAb (A9078)}

Western blot - NEDD4L Rabbit mAb (A9078)

Western blot analysis of extracts of various cell lines, using NEDD4L antibody (A9078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)