Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using NEK7 Rabbit mAb (A19816) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
| Product name | NEK7 Rabbit mAb |
|---|---|
| Catalog No. | A19816 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC2342 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 203-302 of human NEK7 (Q8TDX7). |
|---|---|
| Sequence | MSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | NIMA-related kinase 7; NEK7 |
| Calculated MW | 35kDa |
| Observed MW | 32kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa, MCF7, NIH/3T3, C6, Mouse lung, Mouse liver |
| Cellular location | cytoplasm, microtubule organizing center, nucleoplasm, nucleus, spindle pole, cytoskeleton, centrosome. |
| Customer validation | WB(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A19816? Please let us know so that we can cite the reference in this datasheet.
