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NEK7 Rabbit pAb (A21088)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NEK7 Rabbit pAb (A21088)

Western blot analysis of various lysates using NEK7 Rabbit pAb (A21088) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - NEK7 Rabbit pAb (A21088)

Western blot analysis of various lysates using NEK7 Rabbit pAb (A21088) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameNEK7 Rabbit pAb
Catalog No.A21088
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 257-301 of human NEK7 (NP_598001.1).
SequencePLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTAS
Gene ID
Swiss Prot
Synonyms
Calculated MW35kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, HepG2, Mouse lung, Rat liver
Cellular locationcytoplasm, microtubule organizing center, nucleoplasm, nucleus, spindle pole.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - NEK7 Rabbit pAb (A21088)}

    Western blot - NEK7 Rabbit pAb (A21088)

    Western blot analysis of various lysates using NEK7 Rabbit pAb (A21088) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - NEK7 Rabbit pAb (A21088)}

    Western blot - NEK7 Rabbit pAb (A21088)

    Western blot analysis of various lysates using NEK7 Rabbit pAb (A21088) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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