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NFE2L3 Rabbit pAb (A15761)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NFE2L3 Rabbit pAb (A15761)

Western blot analysis of various lysates using NFE2L3 Rabbit pAb (A15761) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using NFE2L3 Rabbit pAb (A15761) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using NFE2L3 Rabbit pAb (A15761) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using NFE2L3 Rabbit pAb (A15761) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameNFE2L3 Rabbit pAb
Catalog No.A15761
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cap 'n' collar basic-region leucine zipper family of transcription factors. The encoded protein heterodimerizes with small musculoaponeurotic fibrosarcoma factors to bind antioxidant response elements in target genes. This protein is a membrane bound glycoprotein that is targeted to the endoplasmic reticulum and the nuclear envelope. Pseudogenes of this gene are found on chromosomes 16, 17, and 18.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 330-550 of human NFE2L3 (NP_004280.5).
SequenceDPTARTSQSQEPFLQLNSHTTNPEQTLPGTNLTGFLSPVDNHMRNLTSQDLLYDLDINIFDEINLMSLATEDNFDPIDVSQLFDEPDSDSGLSLDSSHNNTSVIKSNSSHSVCDEGAIGYCTDHESSSHHDLEGAVGGYYPEPSKLCHLDQSDSDFHGDLTFQHVFHNHTYHLQPTAPESTSEPFPWPGKSQKIRSRYLEDTDRNLSRDEQRAKALHIPFS
Gene ID
Swiss Prot
SynonymsNRF3; NFE2L3
Calculated MW76kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, RAW264.7
Cellular locationNucleus.

Documents

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ABclonal:Western blot - NFE2L3 Rabbit pAb (A15761)}

Western blot - NFE2L3 Rabbit pAb (A15761)

Western blot analysis of various lysates using NFE2L3 Rabbit pAb (A15761) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)}

Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using NFE2L3 Rabbit pAb (A15761) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)}

Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using NFE2L3 Rabbit pAb (A15761) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)}

Immunohistochemistry - NFE2L3 Rabbit pAb (A15761)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using NFE2L3 Rabbit pAb (A15761) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)