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NFKBIB Rabbit pAb (A5777)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NFKBIB Rabbit pAb (A5777)

Western blot analysis of various lysates using NFKBIB Rabbit pAb (A5777) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

ABclonal:Immunohistochemistry - NFKBIB Rabbit pAb (A5777)

Immunohistochemistry analysis of paraffin-embedded Rat lung using NFKBIB Rabbit pAb (A5777) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NFKBIB Rabbit pAb (A5777)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using NFKBIB Rabbit pAb (A5777) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NFKBIB Rabbit pAb (A5777)

Immunohistochemistry analysis of paraffin-embedded Human kidney cancer using NFKBIB Rabbit pAb (A5777) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameNFKBIB Rabbit pAb
Catalog No.A5777
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2).
SequenceMAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV
Gene ID
Swiss Prot
SynonymsIKBB; TRIP9; NFKBIB
Calculated MW38kDa
Observed MW40-50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7, 293T, Mouse liver, Mouse skeletal muscle
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - NFKBIB Rabbit pAb (A5777)}

Western blot - NFKBIB Rabbit pAb (A5777)

Western blot analysis of various lysates using NFKBIB Rabbit pAb (A5777) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
ABclonal:Immunohistochemistry - NFKBIB Rabbit pAb (A5777)}

Immunohistochemistry - NFKBIB Rabbit pAb (A5777)

Immunohistochemistry analysis of paraffin-embedded Rat lung using NFKBIB Rabbit pAb (A5777) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NFKBIB Rabbit pAb (A5777)}

Immunohistochemistry - NFKBIB Rabbit pAb (A5777)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using NFKBIB Rabbit pAb (A5777) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NFKBIB Rabbit pAb (A5777)}

Immunohistochemistry - NFKBIB Rabbit pAb (A5777)

Immunohistochemistry analysis of paraffin-embedded Human kidney cancer using NFKBIB Rabbit pAb (A5777) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)