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NFS1 Rabbit pAb (A6668)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NFS1 Rabbit pAb (A6668)

Western blot analysis of various lysates using NFS1 Rabbit pAb (A6668) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - NFS1 Rabbit pAb (A6668)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - NFS1 Rabbit pAb (A6668)

Immunofluorescence analysis of C6 cells using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NFS1 Rabbit pAb (A6668)

Immunofluorescence analysis of L929 cells using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NFS1 Rabbit pAb (A6668)

Immunofluorescence analysis of U-251 MG cells using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNFS1 Rabbit pAb
Catalog No.A6668
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 290-457 of human NFS1 (NP_066923.3).
SequenceGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH
Gene ID
Swiss Prot
SynonymsIscS; NIFS; COXPD52; HUSSY-08; NFS1
Calculated MW50kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, HepG2, BxPC-3, Mouse brain, Mouse liver, Mouse heart, Rat brain, Rat liver, Rat heart
Cellular locationCytoplasm, Mitochondrion, Nucleus.

Documents

Certificate of Compliance

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ABclonal:Western blot - NFS1 Rabbit pAb (A6668)}

Western blot - NFS1 Rabbit pAb (A6668)

Western blot analysis of various lysates using NFS1 Rabbit pAb (A6668) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - NFS1 Rabbit pAb (A6668)}

Immunohistochemistry - NFS1 Rabbit pAb (A6668)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - NFS1 Rabbit pAb (A6668)}

Immunofluorescence - NFS1 Rabbit pAb (A6668)

Immunofluorescence analysis of C6 cells using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NFS1 Rabbit pAb (A6668)}

Immunofluorescence - NFS1 Rabbit pAb (A6668)

Immunofluorescence analysis of L929 cells using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NFS1 Rabbit pAb (A6668)}

Immunofluorescence - NFS1 Rabbit pAb (A6668)

Immunofluorescence analysis of U-251 MG cells using NFS1 Rabbit pAb (A6668) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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