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NHLRC1 Rabbit pAb (A6669)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NHLRC1 Rabbit pAb (A6669)

Western blot analysis of lysates from Rat heart, using NHLRC1 Rabbit pAb (A6669) at 1:6000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - NHLRC1 Rabbit pAb (A6669)

Immunohistochemistry analysis of paraffin-embedded Human stomach using NHLRC1 Rabbit pAb (A6669) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - NHLRC1 Rabbit pAb (A6669)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using NHLRC1 Rabbit pAb (A6669) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameNHLRC1 Rabbit pAb
Catalog No.A6669
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a single subunit E3 ubiquitin ligase. Laforin is polyubiquitinated by the encoded protein. Defects in this intronless gene lead to an accumulation of laforin and onset of Lafora disease, also known as progressive myoclonic epilepsy type 2 (EPM2).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NHLRC1 (NP_940988.2).
SequenceMAAEASESGPALHELMREAEISLLECKVCFEKFGHRQQRRPRNLSCGHVVCLACVAALAHPRTLALECPFCRRACRGCDTSDCLPVLHLIELLGSALRQSPAAHRAAPSAPGALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGAIAVLE
Gene ID
Swiss Prot
SynonymsEPM2A; EPM2B; MALIN; bA204B7.2; NHLRC1
Calculated MW42kDa
Observed MW42kDa/45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:7000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat heart
Cellular locationEndoplasmic reticulum, Nucleus.

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ABclonal:Western blot - NHLRC1 Rabbit pAb (A6669)}

Western blot - NHLRC1 Rabbit pAb (A6669)

Western blot analysis of lysates from Rat heart, using NHLRC1 Rabbit pAb (A6669) at 1:6000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - NHLRC1 Rabbit pAb (A6669)}

Immunohistochemistry - NHLRC1 Rabbit pAb (A6669)

Immunohistochemistry analysis of paraffin-embedded Human stomach using NHLRC1 Rabbit pAb (A6669) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - NHLRC1 Rabbit pAb (A6669)}

Immunohistochemistry - NHLRC1 Rabbit pAb (A6669)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using NHLRC1 Rabbit pAb (A6669) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)