제품 > 항체 > 다중클론항체(pAb)

NOD2 Rabbit pAb (A15992)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Human colon using NOD2 Rabbit pAb (A15992) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - NOD2 Rabbit pAb (A15992)

Immunofluorescence analysis of HeLa cells using NOD2 Rabbit pAb (A15992) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using NOD2 Rabbit pAb (A15992) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using NOD2 Rabbit pAb (A15992) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	NOD2 Rabbit pAb
Catalog No.	A15992
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 30-216 of human NOD2 (NP_071445.1).
Sequence	SQEAFQAQRSQLVELLVSGSLEGFESVLDWLLSWEVLSWEDYEGFHLLGQPLSHLARRLLDTVWNKGTWACQKLIAAAQEAQADSQSPKLHGCWDPHSLHPARDLQSHRPAIVRRLHSHVENMLDLAWERGFVSQYECDEIRLPIFTPSQRARRLLDLATVKANGLAAFLLQHVQELPVPLALPLEA
Gene ID
Swiss Prot
Synonyms	CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1; NOD2
Calculated MW	115kDa
Observed MW	100kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse kidney, Rat spleen
Cellular location	basolateral plasma membrane, cell surface, cytoplasm, cytoskeleton, cytosol, Golgi apparatus, mitochondrion, phagocytic vesicle, plasma membrane.
Customer validation	WB(Sus scrofa, Mus musculus, Rattus norvegicus) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구세포 생물학 & 발달 생물학세포사멸카스파제

NOD2 Rabbit pAb images

Immunohistochemistry - NOD2 Rabbit pAb (A15992)

Immunofluorescence - NOD2 Rabbit pAb (A15992)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for NOD2 Rabbit pAb (A15992)

Product:

NOD2 Rabbit pAb

Journal:

Journal of Agricultural and Food Chemistry

Application:

IF:

5.7

Species:

Sus scrofa

PMID:

32208605

Title:

Sodium Butyrate Protects the Intestinal Barrier by Modulating Intestinal Host Defense Peptide Expression and Gut Microbiota after a Challenge with Deoxynivalenol in Weaned Piglets