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NOGO Receptor 1 (RTN4R) Rabbit mAb (A3952)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - NOGO Receptor 1 (RTN4R) Rabbit mAb (A3952)

Western blot analysis of extracts of various cell lines, using NOGO Receptor 1 (RTN4R) Rabbit mAb (A3952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameNOGO Receptor 1 (RTN4R) Rabbit mAb
Catalog No.A3952
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0872
This gene encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human NOGO Receptor 1 (RTN4R) (Q9BZR6).
SequenceRLAANDLQGCAVATGPYHPIWTGRATDEEPLGLPKCCQPDAADKASVLEPGRPASAGNALKGRVPPGDSPPGNGSGPRHINDSPFGTLPGSAEPPLTAVRP
Gene ID
Swiss Prot
SynonymsNGR; NOGOR
Calculated MW51kDa
Observed MW70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA549, U-87MG, Mouse brain
Cellular locationCell membrane, GPI-anchor, Lipid-anchor

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    ABclonal:Western blot - NOGO Receptor 1 (RTN4R) Rabbit mAb (A3952)}

    Western blot - NOGO Receptor 1 (RTN4R) Rabbit mAb (A3952)

    Western blot analysis of extracts of various cell lines, using NOGO Receptor 1 (RTN4R) Rabbit mAb (A3952) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.

    * For research use only. Not for therapeutic or diagnostic purposes.

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