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NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)

Western blot analysis of various lysates using NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)

Immunofluorescence analysis of Neuro-2a cells using NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNOGO Receptor 1 (RTN4R) Rabbit pAb
Catalog No.A5847
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 244-473 of human NOGO Receptor 1 (RTN4R) (NP_075380.1).
SequenceEALAPLRALQYLRLNDNPWVCDCRARPLWAWLQKFRGSSSEVPCSLPQRLAGRDLKRLAANDLQGCAVATGPYHPIWTGRATDEEPLGLPKCCQPDAADKASVLEPGRPASAGNALKGRVPPGDSPPGNGSGPRHINDSPFGTLPGSAEPPLTAVRPEGSEPPGFPTSGPRRRPGCSRKNRTRSHCRLGQAGSGGGGTGDSEGSGALPSLTCSLTPLGLALVLWTVLGPC
Gene ID
Swiss Prot
SynonymsNGR; NOGOR; NOGO Receptor 1 (RTN4R)
Calculated MW51kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesC6, Rat uterus
Cellular locationCell membrane, GPI-anchor, Lipid-anchor.
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)}

    Western blot - NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)

    Western blot analysis of various lysates using NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)}

    Immunofluorescence - NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)

    Immunofluorescence analysis of Neuro-2a cells using NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A5847? Please let us know so that we can cite the reference in this datasheet.