제품 > 항체 > 다중클론항체(pAb)

NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847)

Immunofluorescence analysis of Neuro-2a cells using NOGO Receptor 1 (RTN4R) Rabbit pAb (A5847) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	NOGO Receptor 1 (RTN4R) Rabbit pAb
Catalog No.	A5847
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 244-473 of human NOGO Receptor 1 (RTN4R) (NP_075380.1).
Sequence	EALAPLRALQYLRLNDNPWVCDCRARPLWAWLQKFRGSSSEVPCSLPQRLAGRDLKRLAANDLQGCAVATGPYHPIWTGRATDEEPLGLPKCCQPDAADKASVLEPGRPASAGNALKGRVPPGDSPPGNGSGPRHINDSPFGTLPGSAEPPLTAVRPEGSEPPGFPTSGPRRRPGCSRKNRTRSHCRLGQAGSGGGGTGDSEGSGALPSLTCSLTPLGLALVLWTVLGPC
Gene ID
Swiss Prot
Synonyms	NGR; NOGOR; NOGO Receptor 1 (RTN4R)
Calculated MW	51kDa
Observed MW	66kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	C6, Rat uterus
Cellular location	Cell membrane, GPI-anchor, Lipid-anchor.
Customer validation	WB(Homo sapiens) IHC(Homo sapiens)