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NOS1 Rabbit pAb (A12795)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - NOS1 Rabbit pAb (A12795)

Western blot analysis of extracts of Rat brain, using NOS1 antibody (A12795) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - NOS1 Rabbit pAb (A12795)

Immunofluorescence analysis of L929 cells using NOS1 antibody (A12795) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameNOS1 Rabbit pAb
Catalog No.A12795
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NOS1 (NP_000611.1).
SequenceMEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAP
Gene ID
Swiss Prot
SynonymsNOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
Calculated MW161kDa
Observed MW150kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationCell membrane, Cell projection, Peripheral membrane protein, dendritic spine, sarcolemma
Customer validation

IF(Rattus norvegicus)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - NOS1 Rabbit pAb (A12795)}

Western blot - NOS1 Rabbit pAb (A12795)

Western blot analysis of extracts of Rat brain, using NOS1 antibody (A12795) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - NOS1 Rabbit pAb (A12795)}

Immunofluorescence - NOS1 Rabbit pAb (A12795)

Immunofluorescence analysis of L929 cells using NOS1 antibody (A12795) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12795? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)