제품 > 항체 > 다중클론항체(pAb)

NPHP4 Rabbit pAb (A8934)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NPHP4 Rabbit pAb (A8934)

Western blot analysis of various lysates using NPHP4 Rabbit pAb (A8934) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunofluorescence - NPHP4 Rabbit pAb (A8934)

Immunofluorescence analysis of H9C2 cells using NPHP4 Rabbit pAb (A8934) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NPHP4 Rabbit pAb (A8934)

Immunofluorescence analysis of L929 cells using NPHP4 Rabbit pAb (A8934) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NPHP4 Rabbit pAb (A8934)

Immunofluorescence analysis of U2OS cells using NPHP4 Rabbit pAb (A8934) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNPHP4 Rabbit pAb
Catalog No.A8934
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1247-1426 of human NPHP4 (NP_055917.1).
SequenceRLSLVLRGTQTVRKVRAFTSHPQELKTDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWLVCLCCRQPLISKAFEIMLAAGEGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDSFQVGGGETYTIGLQFAPSQRVGEEEILIYINDHEDKNEEAFCVKVIYQ
Gene ID
Swiss Prot
SynonymsPOC10; SLSN4; NPHP4
Calculated MW158kDa
Observed MW157kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000-1:3000
  • IF 1:50-1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, A-549
Cellular locationCell junction, Cytoplasm, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, tight junction

Documents

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ABclonal:Western blot - NPHP4 Rabbit pAb (A8934)}

Western blot - NPHP4 Rabbit pAb (A8934)

Western blot analysis of various lysates using NPHP4 Rabbit pAb (A8934) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - NPHP4 Rabbit pAb (A8934)}

Immunofluorescence - NPHP4 Rabbit pAb (A8934)

Immunofluorescence analysis of H9C2 cells using NPHP4 Rabbit pAb (A8934) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NPHP4 Rabbit pAb (A8934)}

Immunofluorescence - NPHP4 Rabbit pAb (A8934)

Immunofluorescence analysis of L929 cells using NPHP4 Rabbit pAb (A8934) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NPHP4 Rabbit pAb (A8934)}

Immunofluorescence - NPHP4 Rabbit pAb (A8934)

Immunofluorescence analysis of U2OS cells using NPHP4 Rabbit pAb (A8934) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)