제품 > 항체 > 다중클론항체(pAb)

NPPA Rabbit pAb (A14755)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from Mouse heart, using NPPA Rabbit pAb (A14755) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NPPA Rabbit pAb (A14755)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using NPPA Rabbit pAb (A14755) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat heart using NPPA Rabbit pAb (A14755) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	NPPA Rabbit pAb
Catalog No.	A14755
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NPPA (NP_006163.1).
Sequence	LTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLN
Gene ID
Swiss Prot
Synonyms	ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF; NPPA
Calculated MW	16kDa
Observed MW	16kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Mouse heart
Cellular location	Secreted.
Customer validation	WB(Rattus norvegicus, Mus musculus) PCR(Mus musculus) IP(Mus musculus)

Documents

Certificate of Compliance

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Research Area

NPPA Rabbit pAb images

Western blot - NPPA Rabbit pAb (A14755)

Immunohistochemistry - NPPA Rabbit pAb (A14755)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for NPPA Rabbit pAb (A14755)

Product:

NPPA Rabbit pAb

Journal:

Molecular Medicine Reports

Application:

IF:

2.952

Species:

Rattus norvegicus

PMID:

31702040

Title:

Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism

References for NPPA Rabbit pAb (A14755)

Product:

NPPA Rabbit pAb

Journal:

Molecular Medicine Reports

Application:

IF:

2.952

Species:

Mus musculus

PMID:

31974621

Title:

Effect of SKF‑96365 on cardiomyocyte hypertrophy induced by angiotensin II

References for NPPA Rabbit pAb (A14755)

Product:

NPPA Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Rattus norvegicus

PMID:

35770043

Title:

Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4-PGC-1α-PPARα/PPARγ Signaling in Isolated Beating Rat Atria - PMC

References for NPPA Rabbit pAb (A14755)

Product:

NPPA Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.85

Species:

Rattus norvegicus

PMID:

36565899

Title:

Interaction between A-kinase anchoring protein 5 and protein kinase A mediates CaMKII/HDAC signaling to inhibit cardiomyocyte hypertrophy after hypoxic …

References for NPPA Rabbit pAb (A14755)

Product:

NPPA Rabbit pAb

Journal:

Cell reports. Medicine

Application:

PCR

IF:

16.98

Species:

Mus musculus

PMID:

36809766

Title:

Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease

References for NPPA Rabbit pAb (A14755)

Product:

NPPA Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

6.63

Species:

Rattus norvegicus

PMID:

37952827

Title:

Inhibition of Sema4D attenuates pressure overload-induced pathological myocardial hypertrophy via the MAPK/NF-B/NLRP3 pathways

References for NPPA Rabbit pAb (A14755)

Product:

NPPA Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

WB, IP

IF:

5.486

Species:

Mus musculus

PMID:

35490780

Title:

ERK/RSK-mediated phosphorylation of Y-box binding protein-1 aggravates diabetic cardiomyopathy by suppressing its interaction with deubiquitinase OTUB1