제품 > 항체 > 다중클론항체(pAb)

NPY4R Rabbit pAb (A8143)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - NPY4R Rabbit pAb (A8143)

Western blot analysis of extracts of various cell lines, using NPY4R antibody (A8143) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameNPY4R Rabbit pAb
Catalog No.A8143
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables pancreatic polypeptide receptor activity and peptide hormone binding activity. Involved in G protein-coupled receptor signaling pathway. Located in membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human NPY4R (NP_005963.4).
SequenceMNTSHLLALLLPKSPQGENRSKPLGTPYNFSEHCQDSVDVMVFIVTSYSIETVVGVLGNLCLMCVTVRQKEKANVTNLLI
Gene ID
Swiss Prot
SynonymsY4; PP1; PPYR1; NPY4-R
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, SH-SY5Y, SW480, HT-29
Cellular locationCell membrane, Multi-pass membrane protein

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ABclonal:Western blot - NPY4R Rabbit pAb (A8143)}

Western blot - NPY4R Rabbit pAb (A8143)

Western blot analysis of extracts of various cell lines, using NPY4R antibody (A8143) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)