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NR1I3 Rabbit pAb (A1970)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NR1I3 Rabbit pAb (A1970)

Western blot analysis of various lysates using NR1I3 Rabbit pAb (A1970) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - NR1I3 Rabbit pAb (A1970)

Immunofluorescence analysis of HepG2 cells using NR1I3 Rabbit pAb (A1970) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - NR1I3 Rabbit pAb (A1970)

Immunoprecipitation of NR1I3 in 300 µg extracts from 293T cells transfected with NR1I3 using 3 µg NR1I3 Rabbit pAb (A1970). Western blot analysis was performed using NR1I3 Rabbit pAb (A1970) at 1:1000 dilution.

Overview

Product nameNR1I3 Rabbit pAb
Catalog No.A1970
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. In addition to drug metabolism, the CAR protein is also reported to regulate genes involved in glucose metabolism, lipid metabolism, cell proliferation, and circadian clock regulation. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 103-352 of human NR1I3 (NP_001070948.1).
SequencePVQLSKEQEELIRTLLGAHTRHMGTMFEQFVQFRPPAHLFIHHQPLPTLAPVLPLVTHFADINTFMVLQVIKFTKDLPVFRSLPIEDQISLLKGAAVEICHIVLNTTFCLQTQNFLCGPLRYTIEDGARVSPTVGFQVEFLELLFHFHGTLRKLQLQEPEYVLLAAMALFSPDRPGVTQRDEIDQLQEEMALTLQSYIKGQQRRPRDRFLYAKLLGLLAELRSINEAYGYQIQHIQGLSAMMPLLQEICS
Gene ID
Swiss Prot
SynonymsCAR; CAR1; MB67; NR1I3
Calculated MW40kDa
Observed MW45kDa/40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHT-29, Mouse liver, Rat liver, 293T
Cellular locationCytoplasm, Nucleus, cytoskeleton.
Customer validation

WB(Homo sapiens, Mus musculus, Coturnix japonica)

Documents

Certificate of Compliance

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ABclonal:Western blot - NR1I3 Rabbit pAb (A1970)}

Western blot - NR1I3 Rabbit pAb (A1970)

Western blot analysis of various lysates using NR1I3 Rabbit pAb (A1970) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - NR1I3 Rabbit pAb (A1970)}

Immunofluorescence - NR1I3 Rabbit pAb (A1970)

Immunofluorescence analysis of HepG2 cells using NR1I3 Rabbit pAb (A1970) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - NR1I3 Rabbit pAb (A1970)}

Immunoprecipitation - NR1I3 Rabbit pAb (A1970)

Immunoprecipitation of NR1I3 in 300 µg extracts from 293T cells transfected with NR1I3 using 3 µg NR1I3 Rabbit pAb (A1970). Western blot analysis was performed using NR1I3 Rabbit pAb (A1970) at 1:1000 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)