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NR5A2 Rabbit pAb (A13688)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NR5A2 Rabbit pAb (A13688)

Western blot analysis of various lysates, using NR5A2 Rabbit pAb (A13688) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - NR5A2 Rabbit pAb (A13688)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NR5A2 Rabbit pAb (A13688)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NR5A2 Rabbit pAb (A13688)

Immunofluorescence analysis of paraffin-embedded rat liver using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NR5A2 Rabbit pAb (A13688)

Immunofluorescence analysis of paraffin-embedded mouse liver using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNR5A2 Rabbit pAb
Catalog No.A13688
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-541 of human NR5A2 (NP_995582.1).
SequenceSIPHLILELLKCEPDEPQVQAKIMAYLQQEQANRSKHEKLSTFGLMCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVVHGKEGSIFLVTGQQVDYSIIASQAGATLNNLMSHAQELVAKLRSLQFDQREFVCLKFLVLFSLDVKNLENFQLVEGVQEQVNAALLDYTMCNYPQQTEKFGQLLLRLPEIRAISMQAEEYLYYKHLNGDVPYNNLLIEMLHAKRA
Gene ID
Swiss Prot
SynonymsB1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta; NR5A2
Calculated MW61kDa
Observed MW61kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBxPC-3, Mouse brain
Cellular locationNucleus.

Documents

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ABclonal:Western blot - NR5A2 Rabbit pAb (A13688)}

Western blot - NR5A2 Rabbit pAb (A13688)

Western blot analysis of various lysates, using NR5A2 Rabbit pAb (A13688) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - NR5A2 Rabbit pAb (A13688)}

Immunohistochemistry - NR5A2 Rabbit pAb (A13688)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NR5A2 Rabbit pAb (A13688)}

Immunohistochemistry - NR5A2 Rabbit pAb (A13688)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - NR5A2 Rabbit pAb (A13688)}

Immunofluorescence - NR5A2 Rabbit pAb (A13688)

Immunofluorescence analysis of paraffin-embedded rat liver using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NR5A2 Rabbit pAb (A13688)}

Immunofluorescence - NR5A2 Rabbit pAb (A13688)

Immunofluorescence analysis of paraffin-embedded mouse liver using NR5A2 Rabbit pAb (A13688) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)