제품 > 항체 > 다중클론항체(pAb)

NSG1 Rabbit pAb (A17685)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NSG1 Rabbit pAb (A17685)

Western blot analysis of lysates from Mouse brain, using NSG1 Rabbit pAb (A17685) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameNSG1 Rabbit pAb
Catalog No.A17685
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable clathrin light chain binding activity. Involved in apoptotic process. Located in endoplasmic reticulum.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human NSG1 (NP_055207.1).
SequenceMVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTER
Gene ID
Swiss Prot
SynonymsP21; D4S234; NEEP21; D4S234E; NSG1
Calculated MW
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular location
    ABclonal:Western blot - NSG1 Rabbit pAb (A17685)}

    Western blot - NSG1 Rabbit pAb (A17685)

    Western blot analysis of lysates from Mouse brain, using NSG1 Rabbit pAb (A17685) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)