Western blot - NSUN2 Rabbit mAb (A24132)
Western blot analysis of lysates from Mouse liver using NSUN2 Rabbit mAb (A24132) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:45s.
Immunohistochemistry - NSUN2 Rabbit mAb (A24132)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using NSUN2 Rabbit mAb (A24132) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - NSUN2 Rabbit mAb (A24132)
Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using NSUN2 Rabbit mAb (A24132) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - NSUN2 Rabbit mAb (A24132)
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using NSUN2 Rabbit mAb (A24132) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - NSUN2 Rabbit mAb (A24132)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NSUN2 Rabbit mAb (A24132) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence - NSUN2 Rabbit mAb (A24132)
Confocal imaging of U-251 MG cells using NSUN2 Rabbit mAb (A24132, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - NSUN2 Rabbit mAb (A24132)
Confocal imaging of U-2 OS cells using NSUN2 Rabbit mAb (A24132, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation - NSUN2 Rabbit mAb (A24132)
Immunoprecipitation of NSUN2 in 300 µg extracts from 293T cells using 3 µg NSUN2 Rabbit mAb (A24132). Western blot analysis was performed using NSUN2 Rabbit mAb (A24132) at 1:1000 dilution.