제품 > 항체 > 다중클론항체(pAb)

NSUN5 Rabbit pAb (A5992)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NSUN5 Rabbit pAb (A5992)

Western blot analysis of lysates from Rat brain, using NSUN5 Rabbit pAb (A5992) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - NSUN5 Rabbit pAb (A5992)

Western blot analysis of various lysates using NSUN5 Rabbit pAb (A5992) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NSUN5 Rabbit pAb (A5992)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NSUN5 Rabbit pAb ( A5992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NSUN5 Rabbit pAb (A5992)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using NSUN5 Rabbit pAb ( A5992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NSUN5 Rabbit pAb (A5992)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NSUN5 Rabbit pAb ( A5992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameNSUN5 Rabbit pAb
Catalog No.A5992
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of an evolutionarily conserved family of proteins that may function as methyltransferases. This gene is located in a larger region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. There are two pseudogenes for this gene located in the same region of chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 127-429 of human NSUN5 (NP_060514.1).
SequenceRPGPASQLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAALLKNQGKIFAFDLDAKRLASMATLLARAGVSCCELAEEDFLAVSPSDPRYHEVHYILLDPSCSGSGMPSRQLEEPGAGTPSPVRLHALAGFQQRALCHALTFPSLQRLVYSTCSLCQEENEDVVRDALQQNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFVAVIERVEVPR
Gene ID
Swiss Prot
SynonymsNOL1; p120; NOL1R; NSUN5A; WBSCR20; WBSCR20A; p120(NOL1); NSUN5
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, RD, Rat brain
Cellular locationnucleolus, nucleoplasm

Documents

Certificate of Compliance

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ABclonal:Western blot - NSUN5 Rabbit pAb (A5992)}

Western blot - NSUN5 Rabbit pAb (A5992)

Western blot analysis of lysates from Rat brain, using NSUN5 Rabbit pAb (A5992) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - NSUN5 Rabbit pAb (A5992)}

Western blot - NSUN5 Rabbit pAb (A5992)

Western blot analysis of various lysates using NSUN5 Rabbit pAb (A5992) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NSUN5 Rabbit pAb (A5992)}

Immunohistochemistry - NSUN5 Rabbit pAb (A5992)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NSUN5 Rabbit pAb ( A5992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NSUN5 Rabbit pAb (A5992)}

Immunohistochemistry - NSUN5 Rabbit pAb (A5992)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using NSUN5 Rabbit pAb ( A5992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NSUN5 Rabbit pAb (A5992)}

Immunohistochemistry - NSUN5 Rabbit pAb (A5992)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NSUN5 Rabbit pAb ( A5992) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)