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NTF4 Rabbit pAb (A1081)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NTF4 Rabbit pAb (A1081)

Western blot analysis of various lysates using NTF4 Rabbit pAb (A1081) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NTF4 Rabbit pAb (A1081)

Immunohistochemistry analysis of paraffin-embedded human liver damage using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NTF4 Rabbit pAb (A1081)

Immunohistochemistry analysis of paraffin-embedded rat brain using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NTF4 Rabbit pAb (A1081)

Immunofluorescence analysis of A-549 cells using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NTF4 Rabbit pAb (A1081)

Immunofluorescence analysis of NIH/3T3 cells using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NTF4 Rabbit pAb (A1081)

Immunofluorescence analysis of PC-3 cells using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNTF4 Rabbit pAb
Catalog No.A1081
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human NTF4 (NP_006170.1).
SequenceQPPPSTLPPFLAPEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA
Gene ID
Swiss Prot
SynonymsNT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5; NTF4
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, Mouse testis
Cellular locationSecreted

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Certificate of Compliance

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    ABclonal:Western blot - NTF4 Rabbit pAb (A1081)}

    Western blot - NTF4 Rabbit pAb (A1081)

    Western blot analysis of various lysates using NTF4 Rabbit pAb (A1081) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - NTF4 Rabbit pAb (A1081)}

    Immunohistochemistry - NTF4 Rabbit pAb (A1081)

    Immunohistochemistry analysis of paraffin-embedded human liver damage using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - NTF4 Rabbit pAb (A1081)}

    Immunohistochemistry - NTF4 Rabbit pAb (A1081)

    Immunohistochemistry analysis of paraffin-embedded rat brain using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - NTF4 Rabbit pAb (A1081)}

    Immunofluorescence - NTF4 Rabbit pAb (A1081)

    Immunofluorescence analysis of A-549 cells using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - NTF4 Rabbit pAb (A1081)}

    Immunofluorescence - NTF4 Rabbit pAb (A1081)

    Immunofluorescence analysis of NIH/3T3 cells using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - NTF4 Rabbit pAb (A1081)}

    Immunofluorescence - NTF4 Rabbit pAb (A1081)

    Immunofluorescence analysis of PC-3 cells using NTF4 Rabbit pAb (A1081) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.