제품 > 항체 > 다중클론항체(pAb)

NTSR1 Rabbit pAb (A3054)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - NTSR1 Rabbit pAb (A3054)

Western blot analysis of lysates from DU145 cells, using NTSR1 Rabbit pAb (A3054) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameNTSR1 Rabbit pAb
Catalog No.A3054
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human NTSR1 (NP_002522.2).
SequenceMRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVY
Gene ID
Swiss Prot
SynonymsNTR; NTSR1
Calculated MW46kDa
Observed MW49kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesDU145
Cellular locationCell membrane, Membrane raft, Multi-pass membrane protein

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ABclonal:Western blot - NTSR1 Rabbit pAb (A3054)}

Western blot - NTSR1 Rabbit pAb (A3054)

Western blot analysis of lysates from DU145 cells, using NTSR1 Rabbit pAb (A3054) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)