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NUDT19 Rabbit mAb (A20951)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - NUDT19 Rabbit mAb (A20951)

Western blot analysis of various lysates using NUDT19 Rabbit mAb (A20951) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - NUDT19 Rabbit mAb (A20951)

Western blot analysis of lysates from Rat heart, using NUDT19 Rabbit mAb (A20951) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NUDT19 Rabbit mAb (A20951)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using NUDT19 Rabbit mAb (A20951) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NUDT19 Rabbit mAb (A20951)

Immunofluorescence analysis of K-562 cells using NUDT19 Rabbit mAb (A20951) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNUDT19 Rabbit mAb
Catalog No.A20951
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2938
Predicted to enable magnesium ion binding activity. Predicted to be involved in purine nucleoside bisphosphate metabolic process. Predicted to be located in cytosol and peroxisomal matrix.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-190 of human NUDT19 (A8MXV4).
SequenceHVFSGGVLDAADRSADWLGLFAPHHGPPRFGLGPAPFSRTAFPSLPDTDDHKTDNTGTLPEDVAFRICAVREAFEEAGVLLLRPRTSPPGPAPGPGLALEPPPGLASWRDRVRQDPRHFLRLCAHLDCTPD
Gene ID
Swiss Prot
SynonymsRP2; NUDT19
Calculated MW42kDa
Observed MW45kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, K-562, Jurkat, Rat heart
Cellular locationcytosol, peroxisomal matrix

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ABclonal:Western blot - NUDT19 Rabbit mAb (A20951)}

Western blot - NUDT19 Rabbit mAb (A20951)

Western blot analysis of various lysates using NUDT19 Rabbit mAb (A20951) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - NUDT19 Rabbit mAb (A20951)}

Western blot - NUDT19 Rabbit mAb (A20951)

Western blot analysis of lysates from Rat heart, using NUDT19 Rabbit mAb (A20951) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NUDT19 Rabbit mAb (A20951)}

Immunohistochemistry - NUDT19 Rabbit mAb (A20951)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using NUDT19 Rabbit mAb (A20951) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NUDT19 Rabbit mAb (A20951)}

Immunofluorescence - NUDT19 Rabbit mAb (A20951)

Immunofluorescence analysis of K-562 cells using NUDT19 Rabbit mAb (A20951) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.