제품 > 항체 > 단일클론항체(mAb)

NUP153 Rabbit mAb (A2472)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NUP153 Rabbit mAb (A2472)

Western blot analysis of various lysates using NUP153 Rabbit mAb (A2472) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - NUP153 Rabbit mAb (A2472)

Immunohistochemistry analysis of NUP153 in paraffin-embedded human cervix cancer tissue using NUP153 Rabbit mAb (A2472) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NUP153 Rabbit mAb (A2472)

Immunohistochemistry analysis of NUP153 in paraffin-embedded human tonsil tissue using NUP153 Rabbit mAb (A2472) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NUP153 Rabbit mAb (A2472)

Immunohistochemistry analysis of NUP153 in paraffin-embedded mouse testis tissue using NUP153 Rabbit mAb (A2472) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NUP153 Rabbit mAb (A2472)

Immunofluorescence analysis of U-2 OS cells using NUP153 Rabbit mAb (A2472) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNUP153 Rabbit mAb
Catalog No.A2472
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1922
Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUP153 (P49790).
SequenceMASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCSTDTSEVPRWPENKEDHLVYADEESSNITDGR
Gene ID
Swiss Prot
SynonymsN153; HNUP153; NUP153
Calculated MW154kDa
Observed MW154kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, K-562, Mouse testis
Cellular locationNucleus, Nucleus membrane, nuclear pore complex

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - NUP153 Rabbit mAb (A2472)}

    Western blot - NUP153 Rabbit mAb (A2472)

    Western blot analysis of various lysates using NUP153 Rabbit mAb (A2472) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - NUP153 Rabbit mAb (A2472)}

    Immunohistochemistry - NUP153 Rabbit mAb (A2472)

    Immunohistochemistry analysis of NUP153 in paraffin-embedded human cervix cancer tissue using NUP153 Rabbit mAb (A2472) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - NUP153 Rabbit mAb (A2472)}

    Immunohistochemistry - NUP153 Rabbit mAb (A2472)

    Immunohistochemistry analysis of NUP153 in paraffin-embedded human tonsil tissue using NUP153 Rabbit mAb (A2472) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - NUP153 Rabbit mAb (A2472)}

    Immunohistochemistry - NUP153 Rabbit mAb (A2472)

    Immunohistochemistry analysis of NUP153 in paraffin-embedded mouse testis tissue using NUP153 Rabbit mAb (A2472) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - NUP153 Rabbit mAb (A2472)}

    Immunofluorescence - NUP153 Rabbit mAb (A2472)

    Immunofluorescence analysis of U-2 OS cells using NUP153 Rabbit mAb (A2472) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    Secondary Antibodies (26)