제품 > 항체 > 단일클론항체(mAb)

NUP62 Rabbit mAb (A19271)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NUP62 Rabbit mAb (A19271) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Western blot analysis of lysates from Rat brain, using NUP62 Rabbit mAb (A19271) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - NUP62 Rabbit mAb (A19271)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg NUP62 antibody (A19271). Western blot was performed from the immunoprecipitate using NUP62 antibody (A19271) at a dilution of 1:1000.

Overview

Product name	NUP62 Rabbit mAb
Catalog No.	A19271
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2444

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 423-522 of human NUP62 (P37198).
Sequence	LQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD
Gene ID
Swiss Prot
Synonyms	p62; IBSN; SNDI; NUP62
Calculated MW	53kDa
Observed MW	70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	PC-3, NIH/3T3, Jurkat, Neuro-2a, Rat brain
Cellular location	Cytoplasm, Nucleus, cytoskeleton, nuclear pore complex, spindle pole
Customer validation	WB(Rattus norvegicus)