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NUP98 Rabbit pAb (A0530)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - NUP98 Rabbit pAb (A0530)

Western blot analysis of extracts of various cell lines, using NUP98 antibody (A0530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - NUP98 Rabbit pAb (A0530)

Immunofluorescence analysis of L929 cells using NUP98 antibody (A0530) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameNUP98 Rabbit pAb
Catalog No.A0530
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 621-920 of human NUP98 (NP_624357.1).
SequenceSKPVDENHQQDGDEDSLVSHFYTNPIAKPIPQTPESAGNKHSNSNSVDDTIVALNMRAALRNGLEGSSEETSFHDESLQDDREEIENNSYHMHPAGIILTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVVVYLDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTPLQMALNGKPAPPPQVEKKGQ
Gene ID
Swiss Prot
SynonymsADIR2; NUP96; NUP196; Nup98-96
Calculated MW198kDa
Observed MW105kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-549, LO2, HeLa, Jurkat, HL-60
Cellular locationNucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, nuclear pore complex
Customer validation

IF(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - NUP98 Rabbit pAb (A0530)}

    Western blot - NUP98 Rabbit pAb (A0530)

    Western blot analysis of extracts of various cell lines, using NUP98 antibody (A0530) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - NUP98 Rabbit pAb (A0530)}

    Immunofluorescence - NUP98 Rabbit pAb (A0530)

    Immunofluorescence analysis of L929 cells using NUP98 antibody (A0530) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A0530? Please let us know so that we can cite the reference in this datasheet.

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