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Nanog Rabbit pAb (A3232)

Publications (14) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Nanog Rabbit pAb (A3232)

Western blot analysis of lysates from Rat brain, using Nanog Rabbit pAb (A3232) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Nanog Rabbit pAb (A3232)

Immunohistochemistry analysis of paraffin-embedded Human testicular seminoma using Nanog Rabbit pAb (A3232) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameNanog Rabbit pAb
Catalog No.A3232
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Nanog (NP_079141.2).
SequenceMSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSST
Gene ID
Swiss Prot
SynonymsNANOG; Nanog
Calculated MW35kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat brain
Cellular locationNucleus.
Customer validation

WB(Danio rerio, Mus musculus, Homo sapiens)

IHC(Homo sapiens)

IF(Homo sapiens)

IF(Homo sapiens,Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Nanog Rabbit pAb (A3232)}

Western blot - Nanog Rabbit pAb (A3232)

Western blot analysis of lysates from Rat brain, using Nanog Rabbit pAb (A3232) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Nanog Rabbit pAb (A3232)}

Immunohistochemistry - Nanog Rabbit pAb (A3232)

Immunohistochemistry analysis of paraffin-embedded Human testicular seminoma using Nanog Rabbit pAb (A3232) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3232? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)