제품 > 항체 > 단일클론항체(mAb)

Neurofilament H Rabbit mAb (A19084)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Neurofilament H Rabbit mAb (A19084) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Neurofilament H Rabbit mAb (A19084)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Neurofilament H Rabbit mAb (A19084) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Neurofilament H Rabbit mAb (A19084) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Neurofilament H Rabbit mAb (A19084) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Neurofilament H Rabbit mAb (A19084)

Confocal imaging of frozen sections of mouse brain using Neurofilament H Rabbit mAb (A19084, dilution 1:100) followed by incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunoprecipitation - Neurofilament H Rabbit mAb (A19084)

Immunoprecipitation analysis of 600 μg extracts of Rat brain cells using 3 μg Neurofilament H antibody (A19084). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (A19084) at a dilution of 1:500.

Overview

Product name	Neurofilament H Rabbit mAb
Catalog No.	A19084
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0494

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 550-650 of human Neurofilament H (P12036).
Sequence	AKSPAKEEAKSPPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKAKSPTKEEAKSPE
Gene ID
Swiss Prot
Synonyms	NFH; CMT2CC; Neurofilament H
Calculated MW	112kDa
Observed MW	220kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:3000 IHC-P 1:500 - 1:5000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	Mouse brain, Rat brain
Cellular location	axon, cytoplasm, cytoskeleton, neurofilament, postsynaptic density, postsynaptic intermediate filament cytoskeleton, Schaffer collateral - CA1 synapse.
Customer validation	IF(Sus scrofa, Mus musculus) IHC(Mus musculus) WB(Rattus norvegicus, Homo sapiens)