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Neurofilament H Rabbit pAb (A8442)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Neurofilament H Rabbit pAb (A8442) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Neurofilament H Rabbit pAb (A8442)

Immunofluorescence analysis of C6 cells using Neurofilament H Rabbit pAb (A8442) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded mouse brain using Neurofilament H Rabbit pAb (A8442) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Neurofilament H Rabbit pAb (A8442)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg Neurofilament H antibody (A8442). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (A8442) at a dilution of 1:1000.

Overview

Product name	Neurofilament H Rabbit pAb
Catalog No.	A8442
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 873-1020 of human Neurofilament H (NP_066554.2).
Sequence	KVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK
Gene ID
Swiss Prot
Synonyms	NFH; CMT2CC; Neurofilament H
Calculated MW	112kDa
Observed MW	150kDa/220kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	Mouse brain, Mouse liver, Rat brain, Rat liver
Cellular location	axon, cytoplasm, cytoskeleton, neurofilament, postsynaptic density, postsynaptic intermediate filament cytoskeleton, Schaffer collateral - CA1 synapse.
Customer validation	WB(Rattus norvegicus)