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Neurofilament M Rabbit pAb (A16405)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Neurofilament M Rabbit pAb (A16405)

Western blot analysis of lysates from SH-SY5Y cells, using Neurofilament M Rabbit pAb (A16405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Neurofilament M Rabbit pAb (A16405)

Immunohistochemistry analysis of paraffin-embedded rat brain using Neurofilament M Rabbit pAb (A16405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Neurofilament M Rabbit pAb (A16405)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Neurofilament M Rabbit pAb (A16405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Neurofilament M Rabbit pAb (A16405)

Immunofluorescence analysis of SH-SY5Y cells using Neurofilament M Rabbit pAb (A16405) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNeurofilament M Rabbit pAb
Catalog No.A16405
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human Neurofilament M (NP_005373.2).
SequenceNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKD
Gene ID
Swiss Prot
SynonymsNFM; NEF3; NF-M; Neurofilament M
Calculated MW102kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y
Cellular locationaxon, cytoplasm, intermediate filament cytoskeleton, neurofilament, postsynaptic intermediate filament cytoskeleton
Customer validation

IF(Homo sapiens)

Documents

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ABclonal:Western blot - Neurofilament M Rabbit pAb (A16405)}

Western blot - Neurofilament M Rabbit pAb (A16405)

Western blot analysis of lysates from SH-SY5Y cells, using Neurofilament M Rabbit pAb (A16405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Neurofilament M Rabbit pAb (A16405)}

Immunohistochemistry - Neurofilament M Rabbit pAb (A16405)

Immunohistochemistry analysis of paraffin-embedded rat brain using Neurofilament M Rabbit pAb (A16405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Neurofilament M Rabbit pAb (A16405)}

Immunohistochemistry - Neurofilament M Rabbit pAb (A16405)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Neurofilament M Rabbit pAb (A16405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Neurofilament M Rabbit pAb (A16405)}

Immunofluorescence - Neurofilament M Rabbit pAb (A16405)

Immunofluorescence analysis of SH-SY5Y cells using Neurofilament M Rabbit pAb (A16405) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)