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Neurokinin 1 Receptor (NK1R) Rabbit mAb (A4676)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Neurokinin 1 Receptor (NK1R) Rabbit mAb (A4676)

Western blot analysis of extracts of various cell lines, using Neurokinin 1 Receptor (NK1R) (NK1R) Rabbit mAb (A4676) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - Neurokinin 1 Receptor (NK1R) Rabbit mAb (A4676)

Western blot analysis of extracts of various cell lines, using Neurokinin 1 Receptor (NK1R) (NK1R) Rabbit mAb (A4676) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameNeurokinin 1 Receptor (NK1R) Rabbit mAb
Catalog No.A4676
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1088
This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-407 of human Neurokinin 1 Receptor (NK1R) (NK1R) (P25103).
SequenceYNPIIYCCLNDRFRLGFKHAFRCCPFISAGDYEGLEMKSTRYLQTQGSVYKVSRLETTISTVVGAHEEEPEDGPKATPSSLDLTSNCSSRSDSKTMTESFSFSSNVLS
Gene ID
Swiss Prot
SynonymsSPR; NK1R; NKIR; TAC1R
Calculated MW46kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, SH-SY5Y, Mouse brain, Rat brain, Rat spleen
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Neurokinin 1 Receptor (NK1R) Rabbit mAb (A4676)}

Western blot - Neurokinin 1 Receptor (NK1R) Rabbit mAb (A4676)

Western blot analysis of extracts of various cell lines, using Neurokinin 1 Receptor (NK1R) (NK1R) Rabbit mAb (A4676) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - Neurokinin 1 Receptor (NK1R) Rabbit mAb (A4676)}

Western blot - Neurokinin 1 Receptor (NK1R) Rabbit mAb (A4676)

Western blot analysis of extracts of various cell lines, using Neurokinin 1 Receptor (NK1R) (NK1R) Rabbit mAb (A4676) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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