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Niemann-Pick type C2（NPC2） Rabbit mAb (A23128)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Niemann-Pick type C2（NPC2） Rabbit mAb (A23128) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Niemann-Pick type C2（NPC2） Rabbit mAb (A23128)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Niemann-Pick type C2（NPC2） Rabbit mAb (A23128) at dilution of 1：1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Niemann-Pick type C2（NPC2） Rabbit mAb (A23128) at dilution of 1：1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Niemann-Pick type C2（NPC2） Rabbit mAb (A23128)

Immunofluorescence analysis of paraffin-embedded human lung using Niemann-Pick type C2（NPC2） Rabbit mAb (A23128) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Niemann-Pick type C2（NPC2） Rabbit mAb
Catalog No.	A23128
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59098

Background

This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.

Immunogen information

Immunogen	Recombinant protein of human Niemann-Pick type C2（NPC2）.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	HE1; EDDM1; Niemann-Pick type C2（NPC2）
Calculated MW	17kDa
Observed MW	16-21kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:500- 1:1000 IF 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2, 293F, NIH/3T3
Cellular location	Endoplasmic reticulum, Lysosome, Secreted