제품 > 항체 > 다중클론항체(pAb)

OCIAD1 Rabbit pAb (A11630)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OCIAD1 Rabbit pAb (A11630)

Western blot analysis of lysates from mouse liver, using OCIAD1 Rabbit pAb (A11630) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - OCIAD1 Rabbit pAb (A11630)

Confocal immunofluorescence analysis of U2OS cells using OCIAD1 Rabbit pAb (A11630) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameOCIAD1 Rabbit pAb
Catalog No.A11630
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in several processes, including hematopoietic stem cell homeostasis; positive regulation of receptor signaling pathway via JAK-STAT; and regulation of stem cell differentiation. Located in membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human OCIAD1 (NP_060300.1).
SequenceMNGRADFREPNAEVPRPIPHIGPDYIPTEEERRVFAECNDESFWFRSVPLAATSMLITQGLISKGILSSHPKYGSIPKLILACIMGYFAGKLSYVKTCQEKFKKLENSPLGEALRSGQARRSSPPGHYYQKSKYDSSVSGQSSFVTSPAADNIEMLPHYEPIPFSSSMNESAPTGITDHIVQGPDPNLEESPKRKNITYEELRNKNRESYEVSLTQKTDPSVRPMHERVPKKEVKVNKYGDTWDE
Gene ID
Swiss Prot
SynonymsOCIA; ASRIJ; TPA018; OCIAD1
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver
Cellular locationEndosome.

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ABclonal:Western blot - OCIAD1 Rabbit pAb (A11630)}

Western blot - OCIAD1 Rabbit pAb (A11630)

Western blot analysis of lysates from mouse liver, using OCIAD1 Rabbit pAb (A11630) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - OCIAD1 Rabbit pAb (A11630)}

Immunofluorescence - OCIAD1 Rabbit pAb (A11630)

Confocal immunofluorescence analysis of U2OS cells using OCIAD1 Rabbit pAb (A11630) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)