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OGG1 Rabbit mAb (A4997)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OGG1 Rabbit mAb (A4997)

Western blot analysis of various lysates using OGG1 Rabbit mAb (A4997) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - OGG1 Rabbit mAb (A4997)

Western blot analysis of lysates from Mouse testis, using OGG1 Rabbit mAb (A4997) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - OGG1 Rabbit mAb (A4997)

Immunohistochemistry analysis of paraffin-embedded Rat brain using OGG1 Rabbit mAb (A4997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameOGG1 Rabbit mAb
Catalog No.A4997
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1233
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 246-345 of human OGG1 (O15527).
SequenceVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG
Gene ID
Swiss Prot
SynonymsHMMH; MUTM; OGH1; HOGG1; OGG1
Calculated MW39kDa
Observed MW39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HT-29, U-251MG, Mouse testis
Cellular locationMitochondrion, Nucleus, Nucleus matrix, Nucleus speckle, nucleoplasm.
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - OGG1 Rabbit mAb (A4997)}

Western blot - OGG1 Rabbit mAb (A4997)

Western blot analysis of various lysates using OGG1 Rabbit mAb (A4997) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - OGG1 Rabbit mAb (A4997)}

Western blot - OGG1 Rabbit mAb (A4997)

Western blot analysis of lysates from Mouse testis, using OGG1 Rabbit mAb (A4997) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - OGG1 Rabbit mAb (A4997)}

Immunohistochemistry - OGG1 Rabbit mAb (A4997)

Immunohistochemistry analysis of paraffin-embedded Rat brain using OGG1 Rabbit mAb (A4997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4997? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)