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OSBP Rabbit pAb (A3998)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OSBP Rabbit pAb (A3998)

Western blot analysis of extracts of various cell lines, using OSBP antibody (A3998) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - OSBP Rabbit pAb (A3998)

Immunofluorescence analysis of NIH-3T3 cells using OSBP Rabbit pAb (A3998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameOSBP Rabbit pAb
Catalog No.A3998
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 508-807 of human OSBP (NP_002547.1).
SequenceLEENGYRSLCEQVSHHPPAAAHHAESKNGWTLRQEIKITSKFRGKYLSIMPLGTIHCIFHATGHHYTWKKVTTTVHNIIVGKLWIDQSGEIDIVNHKTGDKCNLKFVPYSYFSRDVARKVTGEVTDPSGKVHFALLGTWDEKMECFKVQPVIGENGGDARQRGHEAEESRVMLWKRNPLPKNAENMYYFSELALTLNAWESGTAPTDSRLRPDQRLMENGRWDEANAEKQRLEEKQRLSRKKREAEAMKATEDGTPYDPYKALWFERKKDPVTKELTHIYRGEYWECKEKQDWSSCPDIF
Gene ID
Swiss Prot
SynonymsOSBP1
Calculated MW89kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HeLa, BT-474, NIH/3T3, Mouse brain, Mouse kidney, Mouse liver, Rat brain
Cellular locationCytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein, cytosol, perinuclear region
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - OSBP Rabbit pAb (A3998)}

Western blot - OSBP Rabbit pAb (A3998)

Western blot analysis of extracts of various cell lines, using OSBP antibody (A3998) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - OSBP Rabbit pAb (A3998)}

Immunofluorescence - OSBP Rabbit pAb (A3998)

Immunofluorescence analysis of NIH-3T3 cells using OSBP Rabbit pAb (A3998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3998? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)