제품 > 항체 > 다중클론항체(pAb)

OSTC Rabbit pAb (A17197)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OSTC Rabbit pAb (A17197)

Western blot analysis of various lysates, using OSTC Rabbit pAb (A17197) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - OSTC Rabbit pAb (A17197)

Western blot analysis of various lysates, using OSTC Rabbit pAb (A17197) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameOSTC Rabbit pAb
Catalog No.A17197
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to contribute to dolichyl-diphosphooligosaccharide-protein glycotransferase activity. Predicted to be involved in protein N-linked glycosylation via asparagine. Part of oligosaccharyltransferase complex.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human OSTC (NP_067050.1).
SequenceYDVIVEPPSVGSMTDEHGHQRPVAFLAYRVNGQYIMEGLASSFLFTMGGLGFIILDRSNAPNIPKLNRFLLLFIGFVCVLLSFFMARVFMRMKLPGYLMG
Gene ID
Swiss Prot
SynonymsDC2; OSTC
Calculated MW17kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F, U-87MG, Mouse liver, Rat liver
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - OSTC Rabbit pAb (A17197)}

    Western blot - OSTC Rabbit pAb (A17197)

    Western blot analysis of various lysates, using OSTC Rabbit pAb (A17197) at 1:400 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - OSTC Rabbit pAb (A17197)}

    Western blot - OSTC Rabbit pAb (A17197)

    Western blot analysis of various lysates, using OSTC Rabbit pAb (A17197) at 1:400 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)