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OTC Rabbit mAb (A25015)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OTC Rabbit mAb (A25015)

Western blot analysis of various lysates using OTC Rabbit mAb (A25015) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - OTC Rabbit mAb (A25015)

Confocal imaging of paraffin-embedded Human liver using OTC Rabbit mAb (A25015, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameOTC Rabbit mAb
Catalog No.A25015
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65231
This nuclear gene encodes a mitochondrial matrix enzyme. The encoded protein is involved in the urea cycle which functions to detoxify ammonia into urea for excretion. Mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human OTC (NP_000522.3).
SequenceLQAATPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGQEEEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPQLQKPKF
Gene ID
Swiss Prot
SynonymsOCTD; OTC1; OTCD; OTCase; OTC
Calculated MW40kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver, Rat liver
Cellular locationMitochondrion matrix

Documents

Certificate of Compliance

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ABclonal:Western blot - OTC Rabbit mAb (A25015)}

Western blot - OTC Rabbit mAb (A25015)

Western blot analysis of various lysates using OTC Rabbit mAb (A25015) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - OTC Rabbit mAb (A25015)}

Immunofluorescence - OTC Rabbit mAb (A25015)

Confocal imaging of paraffin-embedded Human liver using OTC Rabbit mAb (A25015, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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