제품 > 항체 > 다중클론항체(pAb)

OTP Rabbit pAb (A13188)

Publication (0) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OTP Rabbit pAb (A13188)

Western blot analysis of various lysates using OTP Rabbit pAb (A13188) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - OTP Rabbit pAb (A13188)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using OTP Rabbit pAb (A13188) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameOTP Rabbit pAb
Catalog No.A13188
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the homeodomain (HD) family. HD family proteins are helix-turn-helix transcription factors that play key roles in the specification of cell fates. This protein may function during brain development.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human OTP (NP_115485.1).
SequenceMLSHADLLDARLGMKDAAELLGHREAVKCRLGVGGSDPGGHPGDLAPNSDPVEGATLLPGEDITTVGSTPASLAVSAKDPDKQPGPQGGPNPSQAGQQQGQQKQKRHRTRFTPAQLNELERSFAKTHYPD
Gene ID
Swiss Prot
SynonymsOTP
Calculated MW34kDa
Observed MW34-36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Mouse heart, Rat brain, Rat heart
Cellular locationNucleus

Documents

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ABclonal:Western blot - OTP Rabbit pAb (A13188)}

Western blot - OTP Rabbit pAb (A13188)

Western blot analysis of various lysates using OTP Rabbit pAb (A13188) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - OTP Rabbit pAb (A13188)}

Immunohistochemistry - OTP Rabbit pAb (A13188)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using OTP Rabbit pAb (A13188) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.